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产品描述：马血小板衍生生长因子BB(PDGF-BB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的PDGF-BB含量。PDGF - BB即血小板衍生生长因子 - BB，在细胞生长、增殖、迁移及血管生成等生理过程中发挥重要作用。其异常表达与肿瘤、心血管疾病等多种病理状况相关。研究聚焦于其信号通路机制，期望通过干预该靶点开发新的治疗药物和策略。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于科研场景中探究心血管疾病模型、创伤修复机制或干细胞分化调控等相关课题，为体外实验中PDGF-BB的功能研究提供可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PDGF-BB
种属：Equus caballus (Horse)
样本类型：serum, plasma,tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of horse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-91 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 81-87 | |||||
| 1:8 | Average % | 83 | ||||
| Range % | 80-86 | |||||
回收率：
| The recovery of horse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 101-109 | ||||
| EDTA plasma (n=4) | 94 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/ml.OD1OD2AverageCorrected 1002.1322.1062.1191.997 501.7111.7611.7361.614 251.2141.2451.2301.108 12.50.7580.7390.7490.627 6.250.5130.5340.5240.402 3.120.3120.3040.3080.186 1.560.1810.1830.1820.060 00.1210.1230.122 | ||||||
货期：3-5 working days
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