首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠磷酸肌醇3激酶(PI3K)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PIK3CA含量。PIK3CA是一种在多种癌症中常见的癌基因，编码的蛋白是PI3K/AKT信号通路的关键组分。该通路异常激活可促进肿瘤细胞生长、存活和增殖。PIK3CA突变与肿瘤的发生发展密切相关，已成为癌症研究和治疗的重要靶点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，本产品选用高特异性配对抗体构建检测体系，适用于评估实验动物模型中PI3K表达水平的变化，可广泛应用于肿瘤机制研究、代谢性疾病动物模型分析以及药物干预下信号通路活性检测等科研场景。实验操作采用标准ELISA流程，支持多样本类型并行检测，为探索PI3K相关分子机制提供可靠的定量工具，助力基础研究与转化医学中的靶点验证工作。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pik3ca ELISA Kit; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA Kit; PI3-kinase subunit alpha ELISA Kit; PI3K-alpha ELISA Kit; PI3Kalpha ELISA Kit; PtdIns-3-kinase subunit alpha ELISA Kit; EC 2.7.1.153 ELISA Kit; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha ELISA Kit; PtdIns-3-kinase subunit p110-alpha ELISA Kit; p110alpha ELISA Kit; Phosphoinositide-3-kinase catalytic alpha polypeptide ELISA Kit; Serine/threonine protein kinase PIK3CA ELISA Kit; EC 2.7.11.1 ELISA Kit
缩写：PIK3CA
Uniprot No.：P42337
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 95-106 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 96-107 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 83-92 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 89-99 | ||||||
回收率：
| The recovery of mouse PI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 92-101 | |||||
| EDTA plasma (n=4) | 102 | 93-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 15002.7252.8122.7692.625 7502.0202.1652.0931.949 3751.5141.5651.5401.396 187.51.0141.0231.0190.875 940.6140.6650.6400.496 470.3840.3920.3880.244 23.50.2710.2770.2740.130 00.1430.1440.144 | |||||||
货期：3-5 working days
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