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产品描述：人磷脂酶A1成员A(PLA1A) 酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLA1A含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于基础科研领域，如探究PLA1A在脂代谢相关疾病模型中的动态变化、评估其在炎症反应中的作用机制，或用于细胞及动物实验中蛋白表达水平的定量比较分析，为脂质代谢与相关疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AA986889 ELISA Kit; NMD ELISA Kit; OTTHUMP00000215248 ELISA Kit; OTTHUMP00000215249 ELISA Kit; OTTHUMP00000215250 ELISA Kit; Phosphatidylserine specific phospholipase A1 ELISA Kit; Phosphatidylserine specific phospholipase A1alpha ELISA Kit; Phosphatidylserine-specific phospholipase A1 ELISA Kit; Phospholipase A1 member A ELISA Kit; pla1a ELISA Kit; PLA1A_HUMAN ELISA Kit; PS PLA1 ELISA Kit; PS-PLA1 ELISA Kit; PSPLA 1 ELISA Kit; PSPLA1 ELISA Kit
缩写：PLA1A
Uniprot No.：Q53H76
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PLA1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 82-95 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 93-101 | ||||||
| 1:4 | Average % | 99 | |||||
| Range % | 95-106 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 87-99 | ||||||
回收率：
| The recovery of human PLA1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 87-95 | |||||
| EDTA plasma (n=4) | 99 | 95-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 202.3402.3442.3422.237 101.6041.7411.6731.568 50.9360.9680.9520.847 2.50.5760.5540.5650.460 1.250.3530.3250.3390.234 0.6250.2300.2130.2220.117 0.3120.1850.1800.1830.078 00.1040.1060.105 | |||||||
货期：3-5 working days
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