首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠XIIA组分泌型磷脂酶A2(PLA2G12A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLA2G12A含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研实验中定量分析小鼠模型或细胞实验中PLA2G12A的动态变化，可广泛用于探索脂质代谢疾病、动脉粥样硬化、非酒精性脂肪肝等疾病的分子机制，或评估药物干预对PLA2G12A表达的影响，为相关基础研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pla2g12a ELISA Kit; Pla2g12 ELISA Kit; Group XIIA secretory phospholipase A2 ELISA Kit; GXII sPLA2 ELISA Kit; sPLA2-XII ELISA Kit; EC 3.1.1.4 ELISA Kit; Phosphatidylcholine 2-acylhydrolase 12A ELISA Kit
缩写：PLA2G12A
Uniprot No.：Q9EPR2
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PLA2G12A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 8-89 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 82-89 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of mouse PLA2G12A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 83-89 | ||||
| EDTA plasma (n=4) | 90 | 87-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.4102.3242.3672.17820001.6101.6841.6471.45810001.0851.0341.0600.8715000.7000.7320.7160.5272500.4680.4780.4730.2841250.3880.3980.3930.20462.50.2600.2590.2600.07100.1910.1870.189 | ||||||
货期：3-5 working days
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