首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠组织型纤溶酶原激活剂(t-PA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLAT含量。PLAT 即纤溶酶原激活物组织型，是一种丝氨酸蛋白酶。其背景上，在人体纤溶系统中发挥重要作用。研究机制方面，它能特异性地激活血栓部位的纤溶酶原转化为纤溶酶，进而溶解血栓，在血栓性疾病治疗研究中备受关注。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于科研领域中小鼠疾病模型（如血栓性疾病、心血管疾病或肿瘤模型）的机制研究，可定量评估不同实验干预（如药物处理、基因编辑或病理刺激）下生物样本中t - PA的动态变化，为纤溶系统调控及相关分子通路研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Plat ELISA Kit; Tissue-type plasminogen activator ELISA Kit; t-PA ELISA Kit; t-plasminogen activator ELISA Kit; tPA ELISA Kit; EC 3.4.21.68) [Cleaved into: Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B] ELISA Kit
缩写：PLAT
Uniprot No.：P11214
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse t-PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 93-99 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 100-108 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 83-90 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 100-108 | ||||||
回收率：
| The recovery of mouse t-PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-93 | |||||
| EDTA plasma (n=4) | 102 | 98-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
 pg/mlOD1OD2AverageCorrected80002.0762.0002.0381.86540001.4531.5011.4771.30420000.9470.9380.9430.77010000.5640.5590.5620.3895000.3480.3510.3500.1772500.2410.2460.2440.0711250.2050.2000.2030.03000.1720.1740.173 | |||||||
货期：3-5 working days
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