首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠尿激酶型纤溶酶原激活物(uPA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLAU含量。PLAU 即尿激酶型纤溶酶原激活物，在多种生物学过程如细胞迁移、组织重塑等中发挥作用。研究机制主要聚焦于其激活纤溶酶原转变为纤溶酶，参与细胞外基质降解。异常表达与肿瘤侵袭、转移等病理过程密切相关，是潜在治疗靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外培养细胞模型、动物实验样本的检测分析，为科研人员探究肿瘤转移机制、纤维化疾病进程或创伤修复模型提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PlauUrokinase-type plasminogen activator ELISA kit; U-plasminogen activator ELISA kit; uPA ELISA kit; EC 3.4.21.73) [Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B] ELISA kit
缩写：PLAU
Uniprot No.：P06869
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse uPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 96-107 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 90-100 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 89-100 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 92-103 | ||||||
回收率：
| The recovery of mouse uPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 86-97 | |||||
| EDTA plasma (n=4) | 101 | 95-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.5352.4352.4852.3225001.9181.8671.8931.7302501.1701.1431.1570.9941250.6410.6330.6370.47462.50.3980.3850.3920.22931.20.2830.2890.2860.12315.60.2090.2140.2120.04900.1660.1600.163 | |||||||
货期：3-5 working days
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