首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人孤腓肽(OFQ/N)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的PNOC含量。PNOC是一种靶点。它可能参与体内多种生理过程，其相关研究机制或是从神经调节、内分泌等方面入手。通过对PNOC进行深入研究，有望在神经、内分泌等相关疾病的治疗上挖掘新的药物靶点，为开发新疗法提供理论依据。试剂盒检测范围为4.69 pg/mL-300 pg/mL，适用于神经生物学、药理学等领域的基础研究，例如探索疼痛机制、精神疾病模型中的肽类表达变化，或评估细胞实验中OFQ/N的分泌动态。通过可靠的定量结果，为科研人员解析OFQ/N在生理及病理过程中的调控作用提供有力工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/OFQ ELISA Kit; N23K ELISA Kit; N23K/N27K ELISA Kit; Nociceptin, included ELISA Kit; nocistatin ELISA Kit; Npnc1 ELISA Kit; OFQ ELISA Kit; OFQ/N ELISA Kit; Orphanin FQ ELISA Kit; Orphanin FQ2 ELISA Kit; Pnoc ELISA Kit; PNOC_HUMAN ELISA Kit; ppN/OFQ ELISA Kit; PPNOC ELISA Kit; pre-pro-N/OFQ ELISA Kit; prepronociceptin ELISA Kit; pronociceptin ELISA Kit; Propronociceptin ELISA Kit
缩写：OFQ/N
Uniprot No.：Q13519
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：4.69 pg/mL-300 pg/mL
灵敏度：1.17 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human OFQ/N in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 91 | |
| Range % | 80-105 | ||
| 1:2 | Average % | 96 | |
| Range % | 85-114 | ||
| 1:4 | Average % | 93 | |
| Range % | 92-114 | ||
| 1:8 | Average % | 95 | |
| Range % | 80-110 |
回收率：
| The recovery of human OFQ/N spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 80-110 | ||||
| EDTA plasma (n=4) | 95 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 3002.6142.6212.6182.475 1501.8821.8871.8851.742 751.3251.3301.3281.185 37.50.7710.7740.7730.630 18.750.4390.4410.4400.297 9.380.2680.2840.2760.133 4.690.2360.2520.2440.101 00.1380.1480.143 | ||||||
货期：3-5 working days
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