首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠过氧化物酶体增殖因子活化受体γ( PPAR-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PPARG含量。PPARG 是过氧化物酶体增殖物激活受体γ，在脂肪细胞分化、糖脂代谢、炎症调节等多方面发挥重要作用。研究机制上，它可与配体结合后调控下游基因表达，目前是治疗糖尿病等代谢疾病的重要靶点，相关研究聚焦其新功能与药物研发。试剂盒检测范围为0.625 pg/mL-40 pg/mL，广泛应用于肥胖、糖尿病、动脉粥样硬化等代谢相关疾病的动物模型研究，以及脂肪组织、肝脏等器官中PPAR - γ表达调控的机制探索，为药物筛选和基础科研提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pparg ELISA Kit; Nr1c3 ELISA Kit; Peroxisome proliferator-activated receptor gamma ELISA Kit; PPAR-gamma ELISA Kit; Nuclear receptor subfamily 1 group C member 3 ELISA Kit
缩写：PPARG
Uniprot No.：P37238
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 pg/mL-40 pg/mL
灵敏度：0.156 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PPAR-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:2000 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4000 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8000 | Average % | 83 | ||||
| Range % | 80-86 | |||||
回收率：
| The recovery of mouse PPAR-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 107 | 103-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected402.6862.5982.6422.550202.2072.2412.2242.132101.5031.5621.5331.44150.8980.8860.8920.8002.50.4230.4380.4310.3391.250.3040.3260.3150.2230.6250.2010.2150.2080.11600.0910.0930.092 | ||||||
货期：3-5 working days
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