首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠PR结构域锌指蛋白16(PRDM16)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PRDM16含量。PRDM16是重要的转录调控因子，在脂肪细胞分化等生理过程中扮演关键角色。研究发现它能调控棕色脂肪细胞的发育与功能，还和能量代谢、肥胖等密切相关。深入研究其作用机制，有望为代谢性疾病的治疗提供新靶点和策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒适用于科研领域中对小鼠模型中PRDM16蛋白表达的定量分析，可广泛应用于代谢调控、肥胖机制、脂肪细胞分化等基础研究，支持多种生物样本类型检测，为探索PRDM16在生理或病理状态下的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Prdm16 ELISA Kit; Kiaa1675 ELISA Kit; Mel1Histone-lysine N-methyltransferase PRDM16 ELISA Kit; EC 2.1.1.- ELISA Kit; PR domain zinc finger protein 16 ELISA Kit; PR domain-containing protein 16 ELISA Kit; Transcription factor MEL1 ELISA Kit; MDS1/EVI1-like gene 1 ELISA Kit
缩写：PRDM16
Uniprot No.：A2A935
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRDM16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-99 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-106 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of mouse PRDM16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 86-98 | ||||
| EDTA plasma (n=4) | 97 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6512.6832.6672.4695001.9441.9071.9261.7282501.0931.1811.1370.9391250.7440.7320.7380.54062.50.5050.5240.5150.31731.20.3580.3670.3630.16515.60.2770.2680.2730.07500.2010.1950.198 | ||||||
货期：3-5 working days
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