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产品描述：人肽脯氨酰顺反异构酶C(PPIC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PPIC含量。人肽脯氨酰顺反异构酶C（PPIC）是一种在蛋白质折叠和成熟中起关键作用的酶，通过催化脯氨酸酰亚胺肽键的顺反异构化，确保蛋白质正确折叠和成熟，对细胞蛋白质稳态至关重要。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于基础科研中PPIC相关疾病机制探索、细胞模型或动物模型的蛋白表达动态分析，以及药物干预实验中的生物标志物定量评估。可为分子生物学、病理学及药理学研究提供可靠的数据支持，满足科研工作者对PPIC蛋白定量分析的多样化需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cyclophilin C ELISA Kit; CYPC ELISA Kit; MGC3673 ELISA Kit; Parvulin ELISA Kit; Peptidyl prolyl cis trans isomerase C ELISA Kit; Peptidyl-prolyl cis-trans isomerase C ELISA Kit; Peptidylprolyl isomerase C (cyclophilin C) ELISA Kit; peptidylprolyl isomerase C ELISA Kit; PPIase C ELISA Kit; ppiC ELISA Kit; PPIC_HUMAN ELISA Kit; Rotamase C ELISA Kit
缩写：PPIC
Uniprot No.：P45877
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PPIC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 92-104 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 82-98 | ||||||
| 1:4 | Average % | 103 | |||||
| Range % | 98-108 | ||||||
| 1:8 | Average % | 87 | |||||
| Range % | 82-98 | ||||||
回收率：
| The recovery of human PPIC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 85-98 | |||||
| EDTA plasma (n=4) | 111 | 105-115 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.6512.6562.6542.4977502.3222.2512.2872.1303751.6451.6551.6501.493187.51.0020.9740.9880.831940.5870.5440.5660.409470.3890.4010.3950.23823.50.2620.2450.2540.09700.1560.1570.157 | |||||||
货期：3-5 working days
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