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产品描述：小鼠嗜环蛋白/亲环素A(CyPA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PPIA含量。PPIA即亲环蛋白A，是一种在细胞内广泛分布的蛋白质。它参与细胞内的多种生理过程，如蛋白质折叠、信号转导等。其研究机制主要聚焦于与其他蛋白质相互作用构成PPI网络，在炎症、肿瘤等疾病中发挥作用，还可作为潜在治疗靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外实验研究中对CyPA表达水平的动态监测，如炎症机制探索、免疫相关疾病动物模型分析或药物干预实验中生物标志物的定量评估，为科研人员提供稳定可靠的分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ppia ELISA Kit; Peptidyl-prolyl cis-trans isomerase A ELISA Kit; PPIase A ELISA Kit; EC 5.2.1.8 ELISA Kit; Cyclophilin A ELISA Kit; Cyclosporin A-binding protein ELISA Kit; Rotamase A ELISA Kit; SP18) [Cleaved into: Peptidyl-prolyl cis-trans isomerase A ELISA Kit; N-terminally processed] ELISA Kit
缩写：PPIA
Uniprot No.：P17742
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CyPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-93 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-97 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 96-103 | |||||
回收率：
| The recovery of mouse CyPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-97 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.5822.5662.5742.4315001.7821.7761.7791.6362501.0551.0731.0640.9211250.6820.6950.6890.54662.50.4310.4370.4340.29131.20.2920.2820.2870.14415.60.2240.2160.2200.07700.1440.1420.143 | ||||||
货期：3-5 working days
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