首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人亲环蛋白A(PPIA/CYPA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PPIA含量。PPIA 即亲环蛋白 A，是一种在细胞内广泛存在的蛋白质。它参与多种细胞过程，如蛋白质折叠、信号转导等。研究机制上，PPIA 能与多种分子相互作用，影响细胞的生理和病理活动，在炎症、肿瘤等疾病发生发展中可能起重要作用。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于体外培养细胞模型、动物实验或疾病相关生物标志物研究中PPIA/CYPA蛋白表达水平的动态监测，例如在病毒感染机制研究、免疫相关疾病模型或药物干预效果评估等科研场景中，为探索PPIA/CYPA的生物学功能及调控机制提供可靠检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cyclophilin A ELISA Kit; Cyclophilin ELISA Kit; CyclophilinA ELISA Kit; Cyclosporin A binding protein ELISA Kit; Cyclosporin A-binding protein ELISA Kit; CYPA ELISA Kit; CYPH ELISA Kit; Epididymis secretory sperm binding protein Li 69p ELISA Kit; HEL S 69p ELISA Kit; MGC117158 ELISA Kit; MGC12404 ELISA Kit; MGC23397 ELISA Kit; Peptidyl prolyl cis trans isomerase A ELISA Kit; Peptidyl-prolyl cis-trans isomerase A ELISA Kit; Peptidylprolyl isomerase A (cyclophilin A) ELISA Kit; Peptidylprolyl isomerase A ELISA Kit; PPIA ELISA Kit; PPIA protein ELISA Kit; PPIA_HUMAN ELISA Kit; PPIase A ELISA Kit; Rotamase A ELISA Kit; RotamaseA ELISA Kit; T cell cyclophilin ELISA Kit
缩写：PPIA
Uniprot No.：P62937
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PPIA/CYPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of human PPIA/CYPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 84-94 | ||||
| EDTA plasma (n=4) | 95 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.0792.0352.0571.9481001.5941.5761.5851.476500.8490.8310.8400.731250.4460.4390.4430.33412.50.2990.2840.2920.1836.250.2040.1980.2010.0923.120.1530.1580.1560.04700.1100.1080.109 | ||||||
货期：3-5 working days
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