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产品描述：人嗜环蛋白/亲环素B(CyPB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的PPIB含量。PPIB即亲环素B，是一种肽基脯氨酰顺反异构酶。它在细胞内参与蛋白质折叠、转运等过程。研究发现其与多种疾病发病机制有关，如某些肿瘤发展、免疫相关疾病等，可作为潜在治疗靶点来探索新的治疗策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于细胞应激反应研究、免疫调控机制探索以及心血管疾病、肝纤维化等病理模型的生物标志物分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AA408962 ELISA Kit; AA553318 ELISA Kit; AI844835 ELISA Kit; Cphn 2 ELISA Kit; Cphn2 ELISA Kit; Cyclophilin B ELISA Kit; Cyclophilin like protein ELISA Kit; CyP 20b ELISA Kit; CYP S1 ELISA Kit; CYP-S1 ELISA Kit; CYPB ELISA Kit; EC 5.2.1.8 ELISA Kit; MGC14109 ELISA Kit; MGC2224 ELISA Kit; OI9 ELISA Kit; peptidyl prolyl cis trans isomerase B ELISA Kit; Peptidyl prolyl cis trans isomerase B precursor ELISA Kit; Peptidyl-prolyl cis-trans isomerase B ELISA Kit; peptidylprolyl isomerase B (cyclophilin B) ELISA Kit; Peptidylprolyl isomerase B ELISA Kit; PPIase ELISA Kit; PPIase B ELISA Kit; Ppib ELISA Kit; PPIB_HUMAN ELISA Kit; Rotamase ELISA Kit; Rotamase B ELISA Kit; S cyclophilin ELISA Kit; S-cyclophilin ELISA Kit; SCYLP ELISA Kit
缩写：PPIB
Uniprot No.：P23284
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CyPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-85 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-104 | |||||
回收率：
| The recovery of human CyPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-109 | ||||
| EDTA plasma (n=4) | 99 | 93-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5452.4022.4742.35910001.7321.8461.7891.6745001.1871.1641.1761.0612500.6040.6390.6220.5071250.3920.3740.3830.26862.50.2680.2800.2740.15931.250.2010.2080.2050.09000.1180.1120.115 | ||||||
货期：3-5 working days
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