首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠过氧化物氧化还原酶-1(PRDX1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PRDX1含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于研究氧化应激相关疾病（如神经退行性疾病、代谢综合征或炎症模型）中PRDX1的动态变化，也可用于评估抗氧化药物或基因干预手段对PRDX1表达调控的效果，为探索氧化还原平衡机制提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Prdx1 ELISA Kit; Msp23 ELISA Kit; Paga ELISA Kit; Tdpx2 ELISA Kit; Peroxiredoxin-1 ELISA Kit; EC 1.11.1.15 ELISA Kit; Macrophage 23 kDa stress protein ELISA Kit; Osteoblast-specific factor 3 ELISA Kit; OSF-3 ELISA Kit; Thioredoxin peroxidase 2 ELISA Kit; Thioredoxin-dependent peroxide reductase 2 ELISA Kit
缩写：PRDX1
Uniprot No.：P35700
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRDX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of mouse PRDX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-101 | ||||
| EDTA plasma (n=4) | 94 | 91-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.7882.6522.7202.539 101.7641.6931.7291.548 51.1101.0631.0870.906 2.50.7210.6890.7050.524 1.250.3660.3870.3770.196 0.6250.2930.2760.2850.104 0.3120.2290.2350.2320.051 00.1790.1820.181 | ||||||
货期：3-5 working days
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