首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：牛催乳素(PRL/LTH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的PRL含量。催乳素（PRL）是一种由垂体前叶分泌的多肽激素，主要促进乳腺发育和乳汁分泌。其分泌受下丘脑-垂体轴调控，多巴胺等激素可抑制其分泌。催乳素在泌乳过程中起关键作用，其受体为跨膜糖蛋白，参与信号转导。试剂盒检测范围为20 μIU/mL-8000 μIU/mL，本产品适用于畜牧业研究中泌乳机制探索、繁殖周期监测、应激水平评估等科研场景，为优化奶牛饲养管理或乳腺疾病研究提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
缩写：PRL
Uniprot No.：P01239
种属：Bos taurus (Bovine)
样本类型：serum, plasma
检测范围：20 μIU/mL-8000 μIU/mL
灵敏度：20 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 86-104 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-108 | |||||
回收率：
| The recovery of bovine PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-102 | ||||
| EDTA plasma (n=4) | 95 | 89-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2Average 80000.3610.3690.365 15000.6950.7040.700 2501.2051.1991.202 701.5241.5201.522 201.7031.7091.706 02.2452.2432.244 | ||||||
货期：3-5 working days
×
