Horse Prolactin(PRL) ELISA kit

货号: IPD-EL018724HO

Price： ￥3000

48T 96T

数量：

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产品描述：马催乳素(PRL)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的PRL含量。PRL即泌乳素，是腺垂体分泌的多肽激素。其分泌受下丘脑调节。研究机制上，它在乳腺发育、泌乳启动维持等生理过程中发挥重要作用，异常分泌会致月经不调、不孕等，在肿瘤发生发展等方面也有一定关联，是医学研究重要靶点。试剂盒检测范围为2.5 ng/mL-1000 ng/mL，产品经过严格的性能验证，可稳定应用于马科动物繁殖生理、泌乳调控机制、应激状态评估等研究领域，为畜牧科研、动物内分泌研究及生殖健康管理提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
缩写：PRL
Uniprot No.：P12420
种属：Equus caballus (Horse)
样本类型：serum, plasma
检测范围：2.5 ng/mL-1000 ng/mL
灵敏度：2.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-101
1:2	Average %	95
	Range %	85-100
1:4	Average %	91
	Range %	89-99
1:8	Average %	93
	Range %	90-100

回收率：

The recovery of horse PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-105
EDTA plasma (n=4)	97	88-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/mlOD1OD2Average02.1332.1212.1272.51.5161.5011.5098.750.9961.0020.99931.250.5380.5550.547187.50.3410.3390.34010000.1620.1590.161

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