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产品描述：犬催乳素(PRL/LTH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PRL/LTH含量。PRL即催乳素，LTH即促乳素，二者本质相同。此靶点与乳腺发育及泌乳等生理过程密切相关。研究机制上，聚焦于其分泌调控、信号传导通路等，旨在明确异常水平与疾病关联，为高泌乳素血症等疾病诊疗提供依据。试剂盒检测范围为7.5 μIU/mL-3000 μIU/mL，为科研人员研究犬类生殖生理、泌乳机制及内分泌疾病模型提供可靠工具。特别适用于动物繁殖生物学、激素调控机制探索以及药物干预对泌乳功能影响等相关领域的研究，其兼容血清、血浆及多类型组织样本的特点，可满足实验设计中跨组织位点的激素水平对比分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PRL/LTH
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：7.5 μIU/mL-3000 μIU/mL
灵敏度：7.5 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 91-100 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 83-104 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-105 | |||||
回收率：
| The recovery of canine PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-98 | ||||
| EDTA plasma (n=4) | 94 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2Average30000.4050.4080.407562.50.7330.7260.73093.751.1981.1951.19726.251.4791.4841.4827.51.6211.6261.62402.4452.4462.446 | ||||||
货期：3-5 working days
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