Bovine Major prion protein(PRNP) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL018739BO

Price： ￥3000

48T 96T

数量：

大包装询价

产品描述：
牛主要朊病毒蛋白(PRNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PRNP含量。PRNP 编码的蛋白为朊蛋白，在神经系统等中发挥重要作用。其突变或异常聚集可引发朊蛋白病，如疯牛病、克雅氏病等。研究机制主要聚焦于其正常功能维持、异常折叠机制及传播途径，以寻找诊断和治疗方法。试剂盒检测范围为0.01 ng/ml-10 ng/ml，适用于科研场景中PRNP蛋白表达调控、病原体感染机制研究、疫苗开发过程中的生物标志物检测等；为神经退行性疾病模型构建、畜牧业病原体筛查、抗病毒药物筛选等基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
PRNP ELISA Kit; PRP ELISA Kit; Major prion protein ELISA Kit; PrP ELISA Kit; Major scrapie-associated fibril protein 1 ELISA Kit; CD antigen CD230 ELISA Kit
缩写：
PRNP
Uniprot No.：
P10279
种属：
Bos taurus (Bovine)
样本类型：
serum, plasma, tissue homogenates, cell lysates
检测范围：
0.01 ng/ml-10 ng/ml
灵敏度：
0.01 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Neuroscience
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine PRNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-102
1:2	Average %	92
	Range %	86-105
1:4	Average %	88
	Range %	82-98
1:8	Average %	93
	Range %	88-102

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
10	2.603	2.546	2.575	2.399
3.33	1.906	1.933	1.920	1.744
1.11	1.336	1.309	1.323	1.147
0.37	0.871	0.895	0.883	0.707
0.12	0.564	0.595	0.580	0.404
0.04	0.380	0.362	0.371	0.195
0.01	0.266	0.247	0.257	0.081
0	0.171	0.180	0.176