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产品描述:鱼催乳素(PRL/LTH)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的PRL/LTH含量。PRL(泌乳素)和LTH(促乳素,常指PRL)在人体内分泌调节中起关键作用,与乳腺发育和泌乳等生理过程相关。研究聚焦于其在疾病中的机制,如高泌乳素血症发病机制、药物对其水平的影响及信号通路探究,为相关疾病治疗提供方向。试剂盒检测范围为10 μIU/mL-4000 μIU/mL,广泛应用于鱼类繁殖生理学研究、环境内分泌干扰物评估、水产养殖过程中激素水平监测等科研场景,为水生生物内分泌机制探索提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:prl1 ELISA Kit; Prolactin-1 ELISA Kit; PRL-188 ELISA Kit; Prolactin I ELISA Kit
缩写:PRL/LTH
Uniprot No.:P09319
种属:Fish
样本类型:serum, plasma, tissue homogenates
检测范围:10 μIU/mL-4000 μIU/mL
灵敏度:10 μIU/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 95 | ||||
Range % | 91-100 | |||||
1:2 | Average % | 95 | ||||
Range % | 86-104 | |||||
1:4 | Average % | 90 | ||||
Range % | 85-95 | |||||
1:8 | Average % | 96 | ||||
Range % | 92-108 |
回收率:
The recovery of fish PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 96 | 91-102 | ||||
EDTA plasma (n=4) | 95 | 88-105 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
μIU/mlOD1OD2Average 40000.3500.3640.357 7500.6970.7050.701 1251.1961.2031.200 351.5231.5191.521 101.7001.7071.704 02.2442.2422.243 |
货期:3-5 working days
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