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产品描述：人胃泌素释放肽前体(ProGRP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ProGRP含量。ProGRP（胃泌素释放肽前体）是一种在小细胞肺癌（SCLC）中特异性高表达的肿瘤标志物。它作为SCLC发生发展中的生物标志物，可能具有致癌基因的作用，并可能成为潜在的治疗靶点。研究显示，ProGRP在SCLC的诊断、疗效评估和预后预测中具有重要作用。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于肿瘤标志物筛选、疾病机制研究及抗肿瘤药物开发等科研领域，尤其为肺癌相关生物标志物探索、病理模型验证及组织微环境分析提供可靠工具，其优化的检测体系支持多样本类型的高通量检测需求，满足实验室基础研究与转化医学应用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ProGRP
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ProGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 87 | ||||
| Range % | 80-90 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:20 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 86-96 | |||||
回收率：
| The recovery of human ProGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-96 | ||||
| EDTA plasma (n=4) | 97 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.4322.3272.3802.2536001.8941.8861.8901.7633001.2711.2371.2541.1271500.7210.6920.7070.580750.4670.4520.4600.33337.50.3380.3450.3420.21518.750.2380.2250.2320.10500.1290.1250.127 | ||||||
货期：3-5 working days
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