首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠维生素K依赖性蛋白S(PROS1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PROS1含量。PROS1编码的蛋白S是一种维生素K依赖的血浆糖蛋白，作为天然抗凝剂发挥作用。研究机制方面，它可增强活化蛋白C的活性以灭活凝血因子，还能以不依赖活化蛋白C的方式抑制凝血酶原酶复合物的形成，进而调控凝血过程。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于科研场景下的小鼠模型研究，例如探究凝血功能障碍机制、血栓性疾病动物模型的蛋白表达动态变化，或评估肝肾功能异常对PROS1合成的影响。其宽泛的线性范围与多类型样本兼容性（包括组织匀浆）可为心血管疾病、免疫调节及器官特异性研究提供可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pros1 ELISA Kit; ProsVitamin K-dependent protein S ELISA Kit
缩写：PROS1
Uniprot No.：Q08761
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human APOC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:200 | Average % | 92 | |
| Range % | 88-96 | ||
| 1:400 | Average % | 87 | |
| Range % | 84-90 | ||
| 1:800 | Average % | 95 | |
| Range % | 91-99 | ||
| 1:1600 | Average % | 97 | |
| Range % | 92-102 |
回收率：
| The recovery of human APOC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 98 | 94-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 4000.0800.0830.082 2000.1170.1190.118 1000.1770.1720.175 500.2760.2960.286 250.5040.4900.497 12.50.9230.9310.927 6.251.4611.4961.479 02.5392.5872.563 | ||||||
货期：3-5 working days
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