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产品描述：小鼠激肽释放酶1相关肽酶b3(KLK3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KLK3含量。KLK3，即激肽释放酶相关肽酶3，是一种由前列腺上皮细胞分泌的糖蛋白，属于激肽释放酶相关肽酶家族。KLK3在前列腺癌的发生发展中扮演重要角色，其表达水平与前列腺癌的侵袭性相关。研究显示，KLK3基因突变可以预测Gleason评分为7分的前列腺癌患者的侵袭性，为前列腺癌的诊断和治疗提供了新的靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品可广泛应用于小鼠模型的基础研究领域，例如通过检测血清或组织中的 KLK3 表达水平，探索其在前列腺疾病、肿瘤进展或炎症反应中的作用机制，亦可用于药物干预实验中的生物标志物动态监测，为科研人员提供稳定、高效的体外检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：KLK3
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-108 | |||||
回收率：
| The recovery of mouse KLK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-103 | ||||
| EDTA plasma (n=4) | 90 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.4742.3522.4132.242 102.0042.0762.0401.869 51.4251.3541.3901.219 2.50.7640.8010.7830.612 1.250.4820.5120.4970.326 0.6250.3500.3360.3430.172 0.3120.2430.2350.2390.068 00.1690.1730.171 | ||||||
货期：3-5 working days
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