首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠前列腺素-H2 D-异构酶(PTGDS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PTGDS含量。PTGDS 即前列腺素 D 合成酶，在多种生理过程如睡眠调节、炎症反应等中发挥重要作用。研究机制方面，它催化花生四烯酸向前列腺素 D₂转化，其表达和活性异常与多种疾病相关，如神经系统疾病、过敏等，是潜在的药物研发靶点。试剂盒检测范围为4.7 ng/mL-300 ng/mL，本试剂盒可广泛应用于前列腺素代谢通路研究、炎症模型建立、中枢神经系统疾病机制探索等科研场景，尤其适用于需要精确定量组织或体液样本中PTGDS表达水平的实验需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ptgds ELISA Kit; Prostaglandin-H2 D-isomerase ELISA Kit; EC 5.3.99.2 ELISA Kit; Glutathione-independent PGD synthase ELISA Kit; Lipocalin-type prostaglandin-D synthase ELISA Kit; Prostaglandin-D2 synthase ELISA Kit; L-PGDS ELISA Kit; PGD2 synthase ELISA Kit; PGDS2 ELISA Kit
缩写：PTGDS
Uniprot No.：O09114
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：4.7 ng/mL-300 ng/mL
灵敏度：1.2 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PTGDS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of mouse PTGDS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-109 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected3002.4582.3522.4052.2381501.7151.8061.7611.594751.0981.0541.0760.90937.50.6040.6430.6240.45718.80.3560.3710.3640.1979.40.2670.2600.2640.0974.70.2210.2350.2280.06100.1650.1690.167 | ||||||
货期：3-5 working days
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