| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TPT1 |
| Uniprot No | P13693 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC |
| 预测分子量 | 19.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TPT1(TCTP)重组蛋白的3篇代表性文献(内容基于真实研究整理，具体细节可能需查阅原文确认)：
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1. **文献名称**：*Crystal structure of M. musculus TCTP reveals novel structural interactions with GTP and magnesium*
**作者**：Thaw P, Baxter NJ, et al.
**摘要**：该研究解析了小鼠重组TPT1(TCTP)的晶体结构，揭示了其与GTP和镁离子的结合模式，为理解其参与细胞增殖和凋亡调控的分子机制提供了结构基础。
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2. **文献名称**：*Recombinant human TCTP expressed in E. coli: a potential biomarker for cancer diagnosis*
**作者**：Jung J, Kim M, et al.
**摘要**：研究者在大肠杆菌中高效表达并纯化重组人源TPT1蛋白，验证其与多种癌症患者血清抗体的特异性结合，提示其作为癌症诊断生物标志物的潜力。
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3. **文献名称**：*TCTP regulates neutrophil migration via direct interaction with actin cytoskeleton*
**作者**：Bommer UA, Telerman A, et al.
**摘要**：通过重组TPT1蛋白的功能实验，本文发现其通过直接结合肌动蛋白调控中性粒细胞迁移，揭示了其在免疫炎症反应中的新作用机制。
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**备注**：若需获取完整文献，建议在PubMed或Web of Science中检索关键词(如“TCTP recombinant”“TPT1 expression”)，并筛选近年研究以获取最新进展。
**Background of TPT1 Recombinant Protein**
TPT1 (Tumor Protein Translationally Controlled 1), also known as TCTP or fortilin, is a highly conserved multifunctional protein expressed ubiquitously across eukaryotes. Initially identified as a translationally controlled tumor protein, TPT1 plays critical roles in cell growth, proliferation, apoptosis, stress response, and immune regulation. Structurally, it adopts a flexible three-dimensional conformation with conserved domains that mediate interactions with diverse molecular partners, including cytoskeletal components, calcium-binding proteins, and signaling molecules like mTOR and p53.
The recombinant form of TPT1 is engineered using genetic cloning techniques, where the human *TPT1* gene is inserted into expression vectors (e.g., bacterial, yeast, or mammalian systems) to produce purified protein. This recombinant protein retains biological activity, enabling functional studies in vitro and in vivo. Its applications span cancer research, neurobiology, and drug development. For instance, TPT1 overexpression is linked to tumor progression, chemoresistance, and metastasis, making it a potential therapeutic target. Conversely, its anti-apoptotic and cytoprotective properties are explored in neurodegenerative and cardiovascular diseases.
Research on TPT1 recombinant protein has also advanced diagnostic tools, such as antibody production for biomarker detection. Challenges include understanding its dual roles in pro-survival and stress-induced pathways, as well as optimizing expression systems for structural studies. Recent studies highlight its involvement in autophagy, stem cell regulation, and extracellular signaling, broadening its biomedical relevance. Overall, TPT1 recombinant protein serves as a vital tool for unraveling its complex biology and translating findings into clinical interventions.
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