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产品描述：牛甲状旁腺素(PTH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PTH含量。甲状旁腺激素（PTH）是甲状旁腺主细胞分泌的肽类激素。它对钙磷代谢具有关键调节作用。研究机制方面，主要通过作用于骨和肾脏，促进骨钙释放、增加肾小管对钙的重吸收，维持血钙水平稳定，且在骨代谢相关疾病研究中受关注。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于科研场景中牛PTH相关机制研究，如钙代谢调控、骨骼发育分析、营养代谢评估及内分泌疾病模型构建等领域，为畜牧养殖、动物生理学及基础医学研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit; Parathyrin ELISA Kit
缩写：PTH
Uniprot No.：P01268
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 86-99 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 90-110 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 83-99 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 85-105 | ||||||
回收率：
| The recovery of bovine PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 88-98 | |||||
| EDTA plasma (n=4) | 96 | 90-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.6262.6032.6152.49810002.3652.3252.3452.2285001.9861.9921.9891.8722501.5021.4861.4941.3771250.8410.8350.8380.72162.50.4860.4740.4800.36331.250.3710.3750.3730.25600.1150.1190.117 | |||||||
货期：3-5 working days
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