首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人聚合酶Ⅰ和转录本释放因子(PTRF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PTRF含量。PTRF即聚合小窝蛋白结合蛋白，是参与细胞膜上窖蛋白小窝形成的重要成分。其在维持细胞膜稳定性等方面有重要作用。研究发现它与多种疾病发生发展相关，如肿瘤进展等，机制涉及细胞内信号传导、物质转运等过程，是潜在的治疗靶点。试剂盒检测范围为37.5 pg/mL-2400 pg/mL，适用于基础科研领域，例如探究PTRF在肿瘤发生、肌肉疾病或代谢异常等病理模型中的表达变化，或用于细胞信号通路研究中与膜结构动态相关的分子机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：2310075E07Rik ELISA Kit; AW546441 ELISA Kit; Cavin 1 ELISA Kit; Cavin ELISA Kit; Cavin-1 ELISA Kit; CAVIN1 ELISA Kit; CGL4 ELISA Kit; FKSG13 ELISA Kit; FLJ90031 ELISA Kit; MGC118550 ELISA Kit; OTTMUSP00000002049 ELISA Kit; Polymerase I and transcript release factor ELISA Kit; Ptrf ELISA Kit; PTRF_HUMAN ELISA Kit; RNA polymerase I and transcript release factor ELISA Kit; RP23-279L23.6 ELISA Kit; TTF I interacting peptide 12 ELISA Kit
缩写：PTRF
Uniprot No.：Q6NZI2
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：37.5 pg/mL-2400 pg/mL
灵敏度：9.4 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PTRF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 85-92 | ||||||
| 1:2 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:4 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 87-96 | ||||||
回收率：
| The recovery of human PTRF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 83 | 80-86 | |||||
| EDTA plasma (n=4) | 101 | 98-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected24002.3752.3422.3592.25812001.6481.6591.6541.5536000.9600.9320.9460.8453000.5230.4980.5110.4101500.3010.2970.2990.198750.2310.2480.2400.13937.50.1730.1790.1760.07500.1020.1000.101 | |||||||
货期：3-5 working days
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