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产品描述:鸡多肽YY(PYY)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的PYY含量。PYY即肽YY,是肠道分泌的一种激素。它可参与调节食欲和能量平衡,通过与下丘脑等部位的受体结合,抑制食欲、减少食物摄入。目前围绕其作用机制在肥胖、糖尿病等代谢性疾病治疗方面开展了诸多研究,有望开发新型治疗药物。试剂盒检测范围为50 pg/mL-800 pg/mL,本试剂盒可广泛应用于家禽营养学研究中摄食调控机制解析、饲料干预效果评估,以及病理模型中胃肠道功能变化等相关基础研究,为禽类生理代谢研究提供可靠的技术支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:PYY ELISA Kit; Peptide YY-like ELISA Kit; PYY ELISA Kit
缩写:PYY
Uniprot No.:P29203
种属:Gallus gallus (Chicken)
样本类型:serum, plasma, tissue homogenates
检测范围:50 pg/mL-800 pg/mL
灵敏度:50 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Neuroscience
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of chicken PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 92 | ||||
Range % | 86-99 | |||||
1:2 | Average % | 97 | ||||
Range % | 92-103 | |||||
1:4 | Average % | 86 | ||||
Range % | 80-89 | |||||
1:8 | Average % | 90 | ||||
Range % | 83-99 |
回收率:
The recovery of chicken PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 89-98 | ||||
EDTA plasma (n=4) | 93 | 90-97 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2Average 8000.2200.2130.217 4000.3590.3340.347 2000.6070.6210.614 1001.1181.1201.119 502.2282.1892.209 |
货期:3-5 working days
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