首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠再生胰岛衍生蛋白3α(REG3A）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的REG3A含量。REG3A作为一个靶点在研究中备受关注。它在机体的多种生理和病理过程中发挥作用。研究机制上，主要聚焦于其在免疫调节、组织修复及肿瘤发生发展等方面的功能，揭示其信号通路及与其他分子的相互作用，为相关疾病治疗提供思路。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于研究REG3A在糖尿病动物模型中的动态变化、肠道炎症机制探索或组织修复过程中的调控作用，为疾病机制研究、药物疗效评估及分子通路分析提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Reg3a ELISA Kit; Pap2 ELISA Kit; Regenerating islet-derived protein 3-alpha ELISA Kit; REG-3-alpha ELISA Kit; Islet of Langerhans regenerating protein 3 ELISA Kit; Lithostathine 3 ELISA Kit; Pancreatitis-associated protein 2 ELISA Kit; Regenerating islet-derived protein III-alpha ELISA Kit; Reg III-alpha) [Cleaved into: Regenerating islet-derived protein 3-alpha 16.5 kDa form; Regenerating islet-derived protein 3-alpha 15 kDa form] ELISA Kit
缩写：REG3A
Uniprot No.：O09037
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse REG3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 96-104 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 86-95 | ||||||
| 1:8 | Average % | 96 | |||||
| Range % | 91-102 | ||||||
回收率：
| The recovery of mouse REG3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 90-97 | |||||
| EDTA plasma (n=4) | 88 | 84-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected2002.3602.5122.4362.3111001.8311.9871.9091.784501.2521.2541.2531.128250.7470.7660.7570.63212.50.4730.5120.4930.3686.250.3150.3210.3180.1933.120.2190.2170.2180.09300.1240.1260.125 | |||||||
货期：3-5 working days
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