首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠核因子-κB亚基p65亲和肽(NF-κB p65)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RELA含量。RELA（v-rel reticuloendotheliosis viral oncogene homolog A），也称为NF-kappa B p65亚基或转录因子p65，是一种在生长板软骨细胞中表达的转录因子，促进软骨生成。该基因编码NF-κB复合体的主要成分，NF-κB是一种在免疫反应中起作用的保守转录因子系统，同时也参与细胞增殖、发育和凋亡的调节。RELA在免疫系统中发挥核心作用，调节从淋巴细胞和淋巴器官的发育和存活到免疫反应和控制恶变的一系列过程。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中探索炎症性疾病模型、免疫调节机制或神经退行性病变等领域的p65蛋白动态表达分析，例如在动物实验中对感染、氧化应激或药物干预后的信号通路变化进行定量评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Rela ELISA Kit; Nfkb3 ELISA Kit; Transcription factor p65 ELISA Kit; Nuclear factor NF-kappa-B p65 subunit ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 ELISA Kit
缩写：RELA
Uniprot No.：Q04207
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:2 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
回收率：
| The recovery of mouse NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 100-110 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.6592.6002.6302.45110002.0421.9351.9891.8105001.2111.2701.2411.0622500.7360.7240.7300.5511250.3940.4080.4010.22262.50.3070.3110.3090.13031.250.2580.2470.2530.07400.1750.1820.179 | |||||||
货期：3-5 working days
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