首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠络丝蛋白(RELN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RELN含量。RELN（Reelin）蛋白对脑发育和神经元定位至关重要。其研究机制显示，它能与特定受体结合激活下游信号通路，参与调节神经细胞迁移、突触形成等过程。在神经系统疾病研究中，RELN功能异常与精神分裂症、阿尔茨海默病等相关。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于神经科学领域的基础研究，如神经元分化模型、脑发育相关疾病动物模型（如自闭症或精神分裂症模型）中RELN的表达水平分析，或探究其在突触功能调控中的分子机制本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Reln ELISA Kit; Rl ELISA Kit; Reelin ELISA Kit; EC 3.4.21.- ELISA Kit; Reeler protein ELISA Kit
缩写：RELN
Uniprot No.：Q60841
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse RELN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 96-105 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of mouse RELN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 81-94 | ||||
| EDTA plasma (n=4) | 109 | 101-113 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.3452.2302.2882.1062001.4371.4761.4571.2751000.9770.9450.9610.779500.5850.5620.5740.392250.5060.4840.4950.31312.50.3700.3630.3670.1856.250.2880.2810.2850.10300.1840.1800.182 | ||||||
货期：3-5 working days
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