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产品描述：人环形交叉轴突导向受体同源物4(ROBO4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液、尿液样本中的ROBO4含量。ROBO4是一种人环形交叉轴突导向受体同源物，主要在神经系统和血管内皮细胞中表达。它在神经发育中引导神经轴突的路径，同时在血管生成中起重要作用，是肿瘤血管新生和造血干细胞定位的关键靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于血管生物学研究、肿瘤微环境分析、血管生成相关疾病机制探索等科研领域，为研究ROBO4在血管内皮功能调控、病理状态下的表达变化及其信号通路提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ECSM4 ELISA Kit; FLJ20798 ELISA Kit; Magic roundabout ELISA Kit; MGC133352 ELISA Kit; MGC133353 ELISA Kit; MRB ELISA Kit; Robo 4 ELISA Kit; ROBO4 ELISA Kit; ROBO4_HUMAN ELISA Kit; Roundabout homolog 4 ELISA Kit; Roundabout homolog 4 magic roundabout ELISA Kit; Roundabout homolog 4 Precursor ELISA Kit; Roundabout, axon guidance receptor, homolog 4 (Drosophila) ELISA Kit
缩写：ROBO4
Uniprot No.：Q8WZ75
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell culture supernates, urine
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ROBO4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 95-102 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 81-92 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 90-97 | |||||
回收率：
| The recovery of human ROBO4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-95 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.1132.0992.1062.00420001.3641.3211.3431.24110000.8010.7890.7950.6935000.4910.4670.4790.3772500.3030.2920.2980.1961250.2320.2210.2270.12562.50.1690.1570.1630.06100.1050.0990.102 | ||||||
货期：3-5 working days
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