首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠核糖核酸酶，核糖核酸酶A家族，（肝脏，嗜酸性粒细胞源性神经毒素）(RNASE2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RNASE2含量。RNASE2又名核糖核酸酶2，是核糖核酸酶A超家族成员。它在免疫调节等方面有重要作用，可参与宿主防御外来病原体入侵。研究机制上，其可能通过降解RNA发挥生物学功能，在一些炎症及免疫相关疾病研究中受关注。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于探究RNASE2在肝脏疾病机制、嗜酸性粒细胞相关炎症模型或神经免疫调控实验中的动态表达，为动物模型构建、药物干预效果评估及分子通路研究提供可靠工具。满足基础科研领域对RNASE2蛋白水平的精准分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Rnase2 ELISA Kit; Ear4 ELISA Kit; Non-secretory ribonuclease ELISA Kit; EC 4.6.1.18 ELISA Kit; Eosinophil cationic-type ribonuclease 4 ELISA Kit; MR-4 ELISA Kit; Ribonuclease 2 ELISA Kit; RNase 2 ELISA Kit
缩写：RNASE2
Uniprot No.：O35291
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse RNASE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 97-104 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 95-104 | ||||||
回收率：
| The recovery of mouse RNASE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 84-92 | |||||
| EDTA plasma (n=4) | 108 | 104-112 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected2002.4412.5012.4712.3581002.0201.9681.9941.881501.3011.3241.3131.200250.7250.7050.7150.60212.50.4070.4120.4100.2976.250.3040.3110.3080.1953.120.1950.1990.1970.08400.1120.1140.113 | |||||||
货期：3-5 working days
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