首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述:犬松弛肽/松弛素(RLN)酶联免疫试剂盒双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的RLN含量。试剂盒检测范围为3.12 pg/mL-200 pg/mL,适用于犬类生殖生物学研究、妊娠相关疾病模型构建、心血管调节机制探索等科研领域,为动物生殖健康、病理生理学研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:RLN ELISA Kit; Prorelaxin [Cleaved into: Relaxin B chain; Relaxin A chain] ELISA Kit
缩写:RLN
Uniprot No.:Q9TRM8
种属:Canis lupus familiaris (Dog) (Canis familiaris)
样本类型:serum, plasma, tissue homogenates
检测范围:3.12 pg/mL-200 pg/mL
灵敏度:0.78 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of canine RLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 87 | |||||
Range % | 84-90 | ||||||
1:2 | Average % | 95 | |||||
Range % | 91-99 | ||||||
1:4 | Average % | 88 | |||||
Range % | 84-93 | ||||||
1:8 | Average % | 97 | |||||
Range % | 93-100 |
回收率:
The recovery of canine RLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 104 | 101-107 | |||||
EDTA plasma (n=4) | 99 | 94-103 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected2002.3662.5042.4352.2691001.6441.6021.6231.457500.9010.9640.9330.767250.4870.4680.4780.31212.50.3190.3270.3230.1576.250.2610.2680.2650.0993.120.2060.1960.2010.03500.1610.1710.166 |
货期:3-5 working days
×