首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠受体相互作用丝氨酸/苏氨酸激酶3(RIPK3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的RIPK3含量。RIPK3是一种关键的靶点。它在程序性坏死等死亡机制中扮演重要角色。研究发现其参与多种生理和病理过程，如炎症反应、肿瘤发生等。通过对RIPK3作用机制的深入研究，有望为相关疾病的治疗提供新的思路和靶点。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，适用于神经退行性疾病模型、炎症相关机制研究、药物干预效果评估等科研领域，为探索 RIPK3 在细胞死亡信号通路中的功能提供可靠工具，适用于体外实验研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ripk3 ELISA Kit; Rip3 ELISA Kit; Receptor-interacting serine/threonine-protein kinase 3 ELISA Kit; EC 2.7.11.1 ELISA Kit; RIP-like protein kinase 3 ELISA Kit; Receptor-interacting protein 3 ELISA Kit; RIP-3 ELISA Kit; mRIP3 ELISA Kit
缩写：RIPK3
Uniprot No.：Q9QZL0
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：46.88 pg/mL-3000 pg/mL
灵敏度：11.72 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse RIPK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 87-97 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of mouse RIPK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 98 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 30002.6322.8052.7192.555 15002.2352.3652.3002.136 7501.8191.9031.8611.697 3751.2421.3511.2971.133 187.50.6600.7140.6870.523 93.750.4260.4560.4410.277 46.880.2720.2500.2610.097 00.1590.1680.164 | ||||||
货期：3-5 working days
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