首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肾素(Renin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的REN含量。小鼠肾素（Renin）是一种由肾脏分泌的酶，它在生理上通过裂解肝脏产生的血管紧张素原，激活肾素-血管紧张素-醛固酮系统（RAAS），从而调节血压和体液平衡。研究显示，肾素在高血压肾损害中扮演重要角色，其表达与肾纤维化和炎症反应密切相关，可能成为治疗高血压肾损害的新靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于小鼠模型的高通量筛选、肾脏病理机制研究、心血管疾病相关实验以及药物干预后Renin水平变化的动态分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ren1 ELISA Kit; Ren ELISA Kit; Ren-1 ELISA Kit; Renin-1 ELISA Kit; EC 3.4.23.15 ELISA Kit; Angiotensinogenase ELISA Kit; Kidney renin ELISA Kit
缩写：Renin
Uniprot No.：P06281
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 92-108 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of mouse renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 83-98 | ||||
| EDTA plasma (n=4) | 89 | 82-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10003.0202.9522.9862.8535002.4862.2962.3912.2582501.7121.6041.6581.5251251.0310.9580.9950.86262.50.6370.6240.6310.49831.20.4140.3820.3980.26515.60.2640.2480.2560.12300.1370.1290.133 | ||||||
货期：3-5 working days
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