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产品描述：猪肾素(Renin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的REN含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品适用于科研场景中与Renin相关的动物模型研究，如高血压机制探索、肾脏病理分析或药物干预实验，为评估猪心血管及肾脏系统的生理病理变化提供可靠工具，满足基础研究中对目标蛋白精准定量的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FLJ10761, HNFJ2, angiotensin-forming enzyme|angiotensinogenase|renin precursor, renal
缩写：REN
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 85-103 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 85-102 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 83-99 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-110 | ||||||
回收率：
| The recovery of pig renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 82-102 | |||||
| EDTA plasma (n=4) | 94 | 86-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 40001.9731.9971.9851.867 20001.6351.6431.6391.521 10001.2081.1971.2031.085 5000.8050.7960.8010.683 2500.5490.5440.5470.429 1250.3610.3560.3590.241 62.50.2550.2590.2570.139 00.1160.1190.118 | |||||||
货期：3-5 working days
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