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产品描述:人抵抗素样分子β (RELM-β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织培养上清液样本中的RETNLB含量。RELM-β是一种由肠道杯状细胞分泌的蛋白质,在食物过敏中起关键作用。研究表明,RELM-β通过调节肠道微生物群,影响乳杆菌属和另枝菌属细菌的数量,从而破坏食物耐受性。其具体机制涉及与转录激活因子6(STAT6)的结合,以及Th2细胞因子IL-4和IL-13的诱导表达。试剂盒检测范围为47 pg/mL-3000 pg/mL,支持研究者开展炎症性疾病模型评估、代谢综合征机制探索、免疫细胞功能研究等基础科研项目;适用于体外实验体系中RELM-β的动态监测,为肠道屏障功能研究、肺部纤维化模型构建及免疫调控网络解析提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 2 ELISA Kit; CCGR ELISA Kit; Colon and small intestine specific cysteine rich protein ELISA Kit; Colon and small intestine-specific cysteine-rich protein ELISA Kit; Colon carcinoma related gene protein ELISA Kit; Colon carcinoma-related gene protein ELISA Kit; Cysteine rich secreted A12 alpha like protein 1 ELISA Kit; Cysteine rich secreted protein A12 alpha like 1 ELISA Kit; Cysteine rich secreted protein FIZZ2 ELISA Kit; Cysteine-rich secreted protein A12-alpha-like 1 ELISA Kit; Cysteine-rich secreted protein FIZZ2 ELISA Kit; FIZZ1 ELISA Kit; FIZZ2 ELISA Kit; Found in inflammatory zone 1 ELISA Kit; HXCP2 ELISA Kit; RELM beta ELISA Kit; RELMb ELISA Kit; RELMbeta ELISA Kit; Resistin like beta ELISA Kit; Resistin like protein beta ELISA Kit; Resistin-like beta ELISA Kit; RETNB_HUMAN ELISA Kit; RETNL2 ELISA Kit; Retnlb ELISA Kit; XCP2 ELISA Kit
缩写:RETNLB
Uniprot No.:Q9BQ08
种属:Homo sapiens (Human)
样本类型:serum, plasma, cell culture supernates
检测范围:47 pg/mL-3000 pg/mL
灵敏度:11.7 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human RELM-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 85 | |||||
Range % | 80-90 | ||||||
1:2 | Average % | 96 | |||||
Range % | 92-100 | ||||||
1:4 | Average % | 90 | |||||
Range % | 85-94 | ||||||
1:8 | Average % | 92 | |||||
Range % | 88-96 |
回收率:
The recovery of human RELM-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 101 | 95-106 | |||||
EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected30002.2102.3492.2802.12115001.6471.7131.6801.5217501.1291.0921.1110.9523750.7010.6740.6880.529187.50.4380.4570.4480.289940.3520.3690.3610.202470.2240.2350.2300.07100.1560.1620.159 |
货期:3-5 working days
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