首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠环形交叉轴突导向受体同源物4(ROBO4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ROBO4含量。ROBO4是一个重要靶点。其在血管生成与稳定性维持等方面具关键作用。研究机制上，它能参与调节内皮细胞的行为和功能，影响血管的发育和重塑，在肿瘤血管生成等病理过程的研究中受到关注，或为相关疾病治疗提供新方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外培养模型、动物实验样本中ROBO4的动态监测；广泛用于血管生物学、肿瘤微环境、缺血性疾病及炎症相关机制的基础研究，为探索内皮细胞功能调控及病理生理过程提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Robo4 ELISA Kit; Roundabout homolog 4 ELISA Kit
缩写：ROBO4
Uniprot No.：Q8C310
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ROBO4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 81-89 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 95-102 | ||||||
回收率：
| The recovery of mouse ROBO4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 101-109 | |||||
| EDTA plasma (n=4) | 89 | 85-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.5242.6312.5782.40451.9571.9041.9311.7572.51.2401.3041.2721.0981.250.7530.7680.7610.5870.6250.4960.5110.5040.3300.3120.3020.3200.3110.1370.1560.2200.2340.2270.05300.1760.1720.174 | |||||||
货期：3-5 working days
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