| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Troponin T |
| Uniprot No | P45379-11 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-285aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于Troponin T重组蛋白的参考文献概览(注：文献标题与作者为虚拟示例，供参考)：
1. **《High-yield expression and purification of human cardiac Troponin T in E. coli》**
- 作者：Zhang L, et al.
- 摘要：报道了一种利用大肠杆菌重组表达系统高效生产人源心肌肌钙蛋白T(cTnT)的方法，通过优化密码子与纯化工艺，获得高纯度蛋白用于体外诊断试剂开发。
2. **《Structural insights into Troponin T isoform-specific interactions via recombinant protein mutagenesis》**
- 作者：Kawakami H, et al.
- 摘要：通过重组蛋白定点突变技术，解析了Troponin T不同亚型与肌动蛋白结合的关键结构域差异，揭示了其在肌肉收缩调控中的分子机制。
3. **《Recombinant Troponin T as a standardized biomarker in acute myocardial infarction detection》**
- 作者：Müller R, et al.
- 摘要：评估重组cTnT蛋白在心肌梗死诊断中的临床应用，证实其与天然蛋白免疫反应性一致，可用于校准检测试剂盒以提高诊断准确性。
(提示：实际文献需通过PubMed/Google Scholar检索关键词如"recombinant Troponin T expression"、"cardiac biomarker engineering"等获取。)
Troponin T (TnT) is a critical regulatory protein component of the troponin complex, which plays a central role in striated muscle contraction. As one of three subunits (TnT, TnI, TnC), TnT anchors the troponin complex to tropomyosin on thin filaments, enabling calcium-dependent regulation of actin-myosin interactions. In cardiac muscle, cardiac-specific Troponin T (cTnT) is a well-established biomarker for myocardial injury, widely used in clinical diagnostics for conditions like myocardial infarction.
Recombinant Troponin T proteins are engineered using genetic engineering techniques, typically expressed in bacterial (e.g., E. coli) or eukaryotic systems (e.g., mammalian cells) to overcome limitations of tissue-extracted native proteins. This approach ensures consistent production, avoids biological variability, and enables site-specific modifications. Recombinant TnT retains functional epitopes while eliminating contaminating muscle proteins, making it invaluable for antibody development, diagnostic assay calibration, and structural studies.
Research applications include investigating cardiomyopathies caused by TnT mutations (e.g., hypertrophic cardiomyopathy), studying sarcomere dynamics, and developing therapeutic agents targeting cardiac contractility. In diagnostics, recombinant cTnT serves as standardized controls for high-sensitivity cardiac troponin (hs-cTn) assays, essential for early detection of acute coronary syndromes. Recent advances focus on engineering post-translationally modified isoforms and disease-associated mutants to model pathological mechanisms. Challenges remain in replicating native phosphorylation patterns, requiring optimized expression systems. As cardiovascular disease research evolves, recombinant TnT continues to bridge basic science and clinical applications, enabling precision in both mechanistic studies and biomarker quantification.
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