首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人网状蛋白4受体样蛋白2(RTN4RL2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的RTN4RL2含量。RTN4RL2，一种人网状蛋白4受体样蛋白，参与细胞表面受体信号通路、胼胝体发育和负调节。其研究机制涉及神经元轴突导向、髓鞘形成及损伤后神经元保护。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品可广泛应用于神经生物学、细胞信号转导等基础研究领域，支持体外培养细胞模型、动物实验组织样本或体液样本中RTN4RL2蛋白水平的精准定量分析，为探索其在神经再生、疾病机制中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：RTN4RL2 ELISA Kit; NGRH1 ELISA Kit; NGRL3 ELISA Kit; Reticulon-4 receptor-like 2 ELISA Kit; Nogo receptor-like 3 ELISA Kit; Nogo-66 receptor homolog 1 ELISA Kit; Nogo-66 receptor-related protein 2 ELISA Kit; NgR2 ELISA Kit
缩写：RTN4RL2
Uniprot No.：Q86UN3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human RTN4RL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 90-106 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 85-104 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 86-100 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 88-109 | |||||
回收率：
| The recovery of human RTN4RL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 84-107 | ||||
| EDTA plasma (n=4) | 92 | 90-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.0812.0172.0491.94751.7771.7581.7681.6662.51.3371.3261.3321.2301.250.8260.8490.8380.7360.6250.5170.5310.5240.4220.3120.3380.3450.3420.2400.1560.2730.2890.2810.17900.1030.1010.102 | ||||||
货期：3-5 working days
×
