首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人S100钙结合蛋白A8/A9复合物（S100A8/A9）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100A8/A9含量。S100A8/A9是钙结合蛋白S100家族成员，参与炎症反应和细胞生长分化。研究发现，S100A8/A9在多种肿瘤中高表达，可能与肿瘤的发生发展密切相关，其具体机制尚需进一步研究。试剂盒检测范围为1.56 ng/mL-100 ng/mL，该产品适用于体外研究，如炎症机制探索、疾病模型构建、药物干预效果评估或生物标志物相关性分析，为研究感染性病变、肿瘤进展、代谢综合征等病理过程中 S100A8/A9 的动态变化提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：S100A8/A9
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human S100A8/A9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-103 | |||||
回收率：
| The recovery of human S100A8/A9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-89 | ||||
| EDTA plasma (n=4) | 92 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.5452.5412.5432.461501.8501.7261.7881.706251.0211.0011.0110.92912.50.5830.5670.5750.4936.250.3570.3340.3460.2643.120.2050.1980.2020.1201.560.1390.1330.1360.05400.0850.0790.082 | ||||||
货期：3-5 working days
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