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产品描述：人S100钙结合蛋白A8/钙粒蛋白A(S100A8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100A8含量。S100A8是一种钙结合蛋白，属于S100蛋白家族，主要在炎症和免疫反应中发挥调节作用。研究显示，S100A8与多种肿瘤的发生发展密切相关，可能通过调节细胞生长、分化及炎症反应等机制参与肿瘤的侵袭和转移。试剂盒检测范围为1.25 ng/mL-80 ng/mL，适用于基础研究中对炎症相关疾病机制、免疫调控通路或肿瘤生物学行为的探索，例如通过检测临床前模型或体外实验中S100A8的表达变化，评估其在炎症信号传导、免疫细胞活化或肿瘤转移中的作用，为疾病机制研究及药物靶点验证提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：60B8Ag ELISA Kit; AI323541 ELISA Kit; B8Ag ELISA Kit; BEE11 ELISA Kit; CAGA ELISA Kit; Calgranulin-A ELISA Kit; Calprotectin L1L subunit ELISA Kit; Calprotectin; included ELISA Kit; CFAG ELISA Kit; CGLA ELISA Kit; Chemotactic cytokine CP-10 ELISA Kit; CP-10 ELISA Kit; Cystic fibrosis antigen ELISA Kit; L1Ag ELISA Kit; Leukocyte L1 complex light chain ELISA Kit; MA387 ELISA Kit; MIF ELISA Kit; Migration inhibitory factor-related protein 8 ELISA Kit; MRP-8 ELISA Kit; Myeloid-related protein 8 ELISA Kit; Neutrophil cytosolic 7 kDa protein ELISA Kit; NIF ELISA Kit; p8 ELISA Kit; Pro-inflammatory S100 cytokine ELISA Kit; Protein S100-A8 ELISA Kit; S100 calcium binding protein A8 (calgranulin A) ELISA Kit; S100 calcium binding protein A8 ELISA Kit; S100 calcium-binding protein A8 ELISA Kit; S100A8 ELISA Kit; S100A8/S100A9 complex; included ELISA Kit; S10A8_HUMAN ELISA Kit; Urinary stone protein band A ELISA Kit
缩写：S100A8
Uniprot No.：P05109
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human S100A8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 83-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-101 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of human S100A8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-93 | ||||
| EDTA plasma (n=4) | 95 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.9452.9622.9542.800402.5672.5042.5362.382201.6761.7991.7381.584100.8490.8870.8680.71450.4410.4110.4260.2722.50.2630.2510.2570.1031.250.2010.2110.2060.05200.1550.1520.154 | ||||||
货期：3-5 working days
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