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产品描述：人S100-A7A蛋白(S100A7A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、尿液样本中的S100A7A含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，可广泛用于基础医学研究中S100A7A蛋白的功能探索，例如皮肤疾病模型中的炎症标志物检测、肿瘤组织微环境相关蛋白表达分析，或通过体液样本评估生物分子动态变化，为分子机制研究提供灵敏可靠的工具。实验操作兼容常规酶标仪检测，支持多样本类型灵活选择，满足科研场景中复杂样本的检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：S100A7A ELISA Kit; S100A15 ELISA Kit; S100A7L1 ELISA Kit; Protein S100-A7A ELISA Kit; S100 calcium-binding protein A15 ELISA Kit; S100 calcium-binding protein A7-like 1 ELISA Kit; S100 calcium-binding protein A7A ELISA Kit
缩写：S100A7A
Uniprot No.：Q86SG5
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, urine
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human S100A7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 89-96 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 82-97 | ||||||
回收率：
| The recovery of human S100A7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 93-101 | |||||
| EDTA plasma (n=4) | 86 | 81-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.8342.9122.8732.758102.1752.0172.0961.98151.2861.2181.2521.1372.50.6540.6640.6590.5441.250.3970.3860.3920.2770.6250.2490.2420.2460.1310.3120.1670.1620.1650.05000.1150.1140.115 | |||||||
货期：3-5 working days
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