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产品描述：人S100钙结合蛋白A6/钙粒蛋白A(S100A6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100A6含量。S100A6是一个研究靶点。它是S100蛋白家族成员，在细胞生长、分化、凋亡等过程起作用。研究机制方面，其表达变化与肿瘤发生发展、神经系统疾病等密切相关，可作为潜在生物标志物和治疗靶点，有较大研究价值。试剂盒检测范围为31.2 pg/mL-2000 pg/mL，该产品适用于体外实验中对细胞培养上清、疾病模型动物或人类样本中S100A6蛋白表达水平的动态监测，可为探究疾病分子机制、筛选调控靶点或评估药物干预效果提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：2A9 ELISA Kit; 5B10 ELISA Kit; CABP ELISA Kit; CACY ELISA Kit; Calcyclin ELISA Kit; Growth factor inducible protein 2A9 ELISA Kit; Growth factor-inducible protein 2A9 ELISA Kit; MLN 4 ELISA Kit; MLN4 ELISA Kit; OTTHUMP00000015472 ELISA Kit; OTTHUMP00000015473 ELISA Kit; PRA ELISA Kit; PRAGrowth factor inducible protein 2A9 ELISA Kit; Prolactin receptor associated protein ELISA Kit; Prolactin receptor-associated protein ELISA Kit; Protein S100 A6 ELISA Kit; Protein S100-A6 ELISA Kit; S100 A6 ELISA Kit; S100 calcium binding protein A6 (calcyclin) ELISA Kit; S100 calcium binding protein A6 ELISA Kit; S100 calcium-binding protein A6 ELISA Kit; S100A6 ELISA Kit; S10A6_HUMAN ELISA Kit
缩写：S100A6
Uniprot No.：P06703
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/ml-2000 pg/ml
灵敏度：7.81 pg/ml
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human S100A6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||
| Sample | Serum(n=4) | |||
| 1:20 | Average % | 95 | ||
| Range % | 87-99 | |||
| 1:40 | Average % | 101 | ||
| Range % | 94-105 | |||
| 1:80 | Average % | 97 | ||
| Range % | 87-101 | |||
| 1:160 | Average % | 105 | ||
| Range % | 95-109 |
回收率：
| The recovery of human S100A6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 93-106 | |||||
| EDTA plasma (n=4) | 100 | 91-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.6352.5782.6072.512 10002.0362.1932.1152.020 5001.6631.6121.6381.543 2501.1901.1651.1781.083 1250.6750.6860.6810.586 62.50.3850.3890.3870.292 31.20.2330.2370.2350.140 00.0930.0970.095 | |||||||
货期：3-5 working days
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