首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人S100-A4蛋白(S100A4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的S100A4含量。S100A4是一种重要靶点。它属于S100蛋白家族，在细胞内广泛分布。研究发现，S100A4与细胞的增殖、凋亡、迁移等过程密切相关。其异常表达可促进肿瘤的生长、侵袭和转移，目前正被深入研究用于肿瘤等疾病治疗新策略的开发。试剂盒检测范围为0.9 ng/mL-60 ng/mL，该产品可应用于肿瘤微环境研究、纤维化疾病机制探索、药物靶点验证等科研领域，尤其适用于体外模型中S100A4动态表达的定量监测，为分子机制研究和生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：18A2 ELISA Kit; 42A ELISA Kit; calcium Placental protein ELISA Kit; Calvasculin ELISA Kit; CAPL ELISA Kit; Fibroblast specific protein 1 (FSP1) ELISA Kit; Fibroblast specific protein 1 ELISA Kit; Fibroblast specific protein ELISA Kit; FSP1 ELISA Kit; Leukemia multidrug resistance associated protein ELISA Kit; Malignant transformation suppression 1 (MTS1) ELISA Kit; Malignant transformation suppression 1 ELISA Kit; Metastasin ELISA Kit; MTS1 ELISA Kit; OTTHUMP00000015467 ELISA Kit; OTTHUMP00000015468 ELISA Kit; P9KA ELISA Kit; PEL98 ELISA Kit; Placental calcium-binding protein ELISA Kit; Protein Mts1 ELISA Kit; Protein S100 A4 ELISA Kit; Protein S100-A4 ELISA Kit; S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) ELISA Kit; S100 calcium binding protein A4 ELISA Kit; S100 calcium-binding protein A4 ELISA Kit; S100a4 ELISA Kit; S10A4_HUMAN ELISA Kit
缩写：S100A4
Uniprot No.：P26447
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.225 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human S100A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-96 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 82-101 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 93-104 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human S100A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-105 | ||||
| EDTA plasma (n=4) | 100 | 95-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 602.6542.5872.6212.471 302.2162.1152.1662.016 151.3691.4061.3881.238 7.50.6890.7150.7020.552 3.750.3850.3670.3760.226 1.80.2880.2790.2840.134 0.90.2160.2060.2110.061 00.1560.1440.150 | ||||||
货期：3-5 working days
×
