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产品描述：人S100-A2蛋白(S100A2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的S100A2含量。S100A2是一种钙结合蛋白，具有抑制肿瘤生长的潜力。研究发现，S100A2在多种恶性肿瘤中表达降低，可能与肿瘤的发生发展及预后密切相关。其研究机制涉及调节细胞增殖、分化、凋亡等过程，并可能通过影响葡萄糖代谢和细胞信号传导等途径发挥抑癌作用。试剂盒检测范围为1.56 ng/mL-100 ng/mL，本试剂盒通过特异性抗体对实现高灵敏度检测，适用于肿瘤机制研究、药物开发疗效评估及生物标志物探索等科研领域，可为体外实验提供精准的蛋白浓度数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CAN19 ELISA Kit; MGC111539 ELISA Kit; Protein S 100L ELISA Kit; Protein S-100L ELISA Kit; Protein S100 A2 ELISA Kit; Protein S100-A2 ELISA Kit; Protein S100L ELISA Kit; S100 A2 ELISA Kit; S100 calcium binding protein A2 ELISA Kit; S100 calcium-binding protein A2 ELISA Kit; S100A2 ELISA Kit; S100L ELISA Kit; S10A2_HUMAN ELISA Kit
缩写：S100A2
Uniprot No.：P29034
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human S100A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 105 | ||||
| Range % | 99-110 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human S100A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 83-94 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1001.9872.0212.0041.802501.5561.5321.5441.342251.1011.0161.0590.85712.50.6870.6960.6920.4906.250.4630.4310.4470.2453.120.3240.3120.3180.1161.560.2640.2710.2680.06600.1990.2040.202 | ||||||
货期：3-5 working days
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