Canine S100 calcium binding protein A12/Calgranulin-C(S100A12) ELISA Kit

大包装询价

货号: IPD-E17221c

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
犬S100钙结合蛋白A12/钙粒蛋白C(S100A12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的S100A12含量。S100A12是一个靶点。它属于S100蛋白家族，在炎症反应等生理病理过程中起重要作用。研究机制方面，其可与细胞表面受体结合，激活多条信号通路，参与免疫调节、细胞增殖和迁移等过程，是疾病诊断和治疗研究的潜在靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品适用于实验动物模型中炎症相关疾病的机制研究、药物干预效果评估以及细胞实验中蛋白表达水平动态监测，其多类型样本兼容性为组织微环境分析、体外细胞模型研究及体液生物标志物探索提供可靠工具。为科研工作者解析S100A12在犬类疾病模型中的功能机制提供高效检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
S100A12
Uniprot No.：
F1PMK3
种属：
Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：
serum, plasma, cell culture supernates, tissue homogenates
检测范围：
0.625 ng/mL-40 ng/mL
灵敏度：
0.156 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine S100A12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	96
	Range %	93-99
1:4	Average %	87
	Range %	82-91
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of canine S100A12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	97	90-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average	Corrected
40	1.928	2.035	1.982	1.853
20	1.460	1.568	1.514	1.385
10	0.949	0.928	0.939	0.810
5	0.565	0.578	0.572	0.443
2.5	0.377	0.398	0.388	0.259
1.25	0.252	0.264	0.258	0.129
0.625	0.196	0.198	0.197	0.068
0	0.132	0.126	0.129

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.