首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠S100-A4蛋白(S100A4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的S100A4含量。S100A4是一个备受关注的靶点。它在细胞的增殖、迁移等过程中扮演重要角色。目前研究发现其在多种肿瘤的发生、发展和转移中起促进作用，可能通过调节细胞骨架重构等机制发挥功能，有望成为肿瘤治疗等领域新的药物作用靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品适用于肿瘤微环境研究、纤维化疾病模型评估、炎症相关信号通路解析等基础研究领域，同时可为药物干预效果提供定量分析支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：S100a4 ELISA Kit; Capl ELISA Kit; Mts1 ELISA Kit; Protein S100-A4 ELISA Kit; Metastasin ELISA Kit; Metastatic cell protein ELISA Kit; PEL98 ELISA Kit; Placental calcium-binding protein ELISA Kit; Protein 18A2 ELISA Kit; Protein Mts1 ELISA Kit; S100 calcium-binding protein A4 ELISA Kit
缩写：S100A4
Uniprot No.：P07091
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse S100A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:200 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:400 | Average % | 103 | ||||
| Range % | 99-108 | |||||
| 1:800 | Average % | 98 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of mouse S100A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.4182.5242.4712.277 20001.8541.9171.8861.692 10001.3601.2941.3271.133 5000.9120.8930.9030.709 2500.6590.6270.6430.449 1250.4010.4210.4110.217 62.50.3120.2970.3050.111 00.1970.1900.194 | ||||||
货期：3-5 working days
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