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产品描述：人S100钙结合蛋白A9/钙粒蛋白B(S100A9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100A9含量。S100A9是一个备受关注的靶点。它在多种生理和病理过程中有重要作用，尤其与炎症反应、免疫调节密切相关。其研究机制主要聚焦于与其他蛋白相互作用，影响细胞信号传导通路，在肿瘤、炎症性疾病等方面的潜在治疗价值是研究热点。试剂盒检测范围为4.69 ng/mL-300 ng/mL，该产品为研究S100A9在炎症信号通路、肿瘤免疫微环境中的功能机制提供可靠工具，适用于基础科研中疾病模型评估、药物干预效果分析或生物标志物筛选等场景，帮助解析S100A9在相关疾病中的分子作用及潜在应用价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Leukocyte L1 complex heavy chain ELISA Kit; 60B8AG ELISA Kit; CAGB ELISA Kit; Calgranulin B ELISA Kit; Calgranulin-B ELISA Kit; Calprotectin L1H subunit ELISA Kit; CFAG ELISA Kit; CGLB ELISA Kit; Cystic fibrosis antigen B ELISA Kit; L1AG ELISA Kit; Leukocyte L1 complex heavy chain ELISA Kit; LIAG ELISA Kit; MAC387 ELISA Kit; MIF ELISA Kit; Migration inhibitory factor related protein 14 ELISA Kit; Migration inhibitory factor-related protein 14 ELISA Kit; MRP 14 ELISA Kit; MRP-14 ELISA Kit; MRP14 ELISA Kit; Myeloid-related protein 14 ELISA Kit; NIF ELISA Kit; OTTHUMP00000015331 ELISA Kit; p14 ELISA Kit; Protein S100-A9 ELISA Kit; S100 A9 ELISA Kit; S100 calcium binding protein A9 ELISA Kit; S100 calcium binding protein A9 calgranulin B ELISA Kit; S100 calcium-binding protein A9 ELISA Kit; S100A9 ELISA Kit; S10A9_HUMAN ELISA Kit
缩写：S100A9
Uniprot No.：P06702
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：4.69 ng/mL-300 ng/mL
灵敏度：1.17 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human S100A9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 94-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of human S100A9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-95 | ||||
| EDTA plasma (n=4) | 96 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected3002.6152.5562.5862.4451501.8411.7841.8131.672751.2631.2511.2571.11637.50.7570.7390.7480.60718.750.4610.4450.4530.3129.380.2880.2730.2810.1404.690.2070.1980.2030.06200.1420.1400.141 | ||||||
货期：3-5 working days
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