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产品描述：人二胺乙酰转移酶1(SAT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SAT1含量。SAT1即精胺/亚精胺 N1 - 乙酰基转移酶1，是调控细胞内多胺水平的关键限速酶。研究发现，它通过调节多胺的代谢及运输等参与多种生理和病理过程，与细胞增殖、凋亡等密切相关，在肿瘤等疾病中展现出潜在的研究与应用价值。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础研究领域探索SAT1在疾病模型中的功能机制、评估药物干预对多胺代谢通路的影响，或作为生物标志物用于相关分子机制研究，为细胞生物学、病理学及药理学研究提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DC21 ELISA Kit; Diamine acetyltransferase 1 ELISA Kit; Diamine N acetyltransferase 1 ELISA Kit; EC 2.3.1.57 ELISA Kit; KFSD ELISA Kit; KFSDX ELISA Kit; Polyamine N acetyltransferase 1 ELISA Kit; Polyamine N-acetyltransferase 1 ELISA Kit; Putrescine acetyltransferase ELISA Kit; SAT ELISA Kit; SAT1 ELISA Kit; SAT1_HUMAN ELISA Kit; Spermidine/spermine N(1) acetyltransferase 1 ELISA Kit; Spermidine/spermine N(1)-acetyltransferase 1 ELISA Kit; Spermidine/spermine N1 acetyltransferase 1 ELISA Kit; Spermidine/spermine N1 acetyltransferase alpha ELISA Kit; SSAT 1 ELISA Kit; SSAT ELISA Kit; SSAT-1 ELISA Kit
缩写：SAT1
Uniprot No.：P21673
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SAT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 93-102 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 82-92 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of human SAT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-92 | ||||
| EDTA plasma (n=4) | 100 | 93-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5602.5132.5372.40851.6231.5871.6051.4762.51.0941.0681.0810.9521.250.6580.6480.6530.5240.6250.4370.4510.4440.3150.3120.2730.2660.2700.1410.1560.1980.1880.1930.06400.1280.1300.129 | ||||||
货期：3-5 working days
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